Study of Apoptotic Properties of Silver Nanoparticles Green Synthesized Using Amaranthus cruentus Extract in Breast Cancer Cells (MDA)

Introduction: There are different treatment methods for cancer therapy, including surgical, anatomy, biopsy, chemotherapy and radiotherapy, but the side effects of these methods as well as their inefficiency for complete omission of cancer have led researchers to find a way to safety remove cells and cancerous tumors. The aim of this study was evaluation of cytotoxicity effects and apoptotic properties of silver nanoparticles synthesized by Amaranthus cruentus in breast cancer cells. Methods: In this study the green method was used to synthesize silver nanoparticles by using Amaranthus cruentus extract. Then, MDA-MB-231 cells were treated with different concentrations of nanoparticles. The cytotoxicity effects of silver nanoparticles and Bax and Bcl-2 genes expressions were analyzed by using MTT assay and real-time PCR respectively. Results: The results of MTT assay showed silver nanoparticles inhibit the proliferation of MDA cells depending on the concentration and time. Concentration 47μg/ml in 24h, 45 μg/ml in 48h and 31 μg/ml in 72h caused the death of 50% of MDA cells. Also, the result of real-Time PCR for treated cells showed Bax gene expression was increased, while the expression of the Bcl-2 gene was decreased. Conclusions: Silver nanoparticles cause apoptosis in MDA cells and using these nanoparticles can be considered as a promising strategy in the treatment of breast cancer.